Milan Analytica AG Switzerland
 

Sperm purification and Isolation by magnetic beads

Bulk Biologicals


Product Description

Annexin AV, Annexin V

marker for apoptosis, beads used to remove programmed death sperm cells

Ubiquitin

tag for defective sperm cells, beads used to remove damaged sperm cells

PSA Pisum sativum agglutinin (lectin)

marker for sperm cells with damaged acrosome

WGA Wheat Germ agglutinin (lectin), Triticum vulgaris

binds to negative charge at sialic acid sites and N-acetyl Glucosamine, GalNAc

Con A, Concanavalin A (lectin), Conavalia ensiformis

binds to alpha mannose fertile sperm surface marker increases with capacitation

SBA soybean agglutinin (lectin), Glycine max

binds to beta N-acetylgalactosamine, GalNAc

PNA peanut agglutinin (lectin), Arachis hypogaea

binds to ß-D-Gal(1-3)-D-galNAc

UEA 1, Ulex europaeus agglutinin (lectin)

binds to alpha fucose

DBA Dolichos biflorus agglutinin

binds to alpha N-acetylgalactosamine , GalNAc

Jacalin, jac, Artocarpus integrifolia

binds to terminal alpha Galactose

Silica

glass magnetic beads bind to non motile sperm

Mannose

surface marker for fertile sperm

Clusterin

detection of freezing abnormalities, associated with the clearance of cellular debris and apoptosis

CD52

major maturation antigen, may be a target for sperm antibodies It is a glycosylphosphatidylinositol (GPI)--anchored antigen expressed within the male genital tract and on all lymphocytes

anti human IgA

detection and removal of sperm coated antibody

Caspase 3

cleaves PARP (poly ADPribose polymerase), apoptotic marker

Caspase 7

executioner caspase responsible for cell death, apoptotic marker

Caspase 9

initiator caspase, starts the caspase cascade, apoptotic marker

AIF, apoptosis inducing factor

induces apoptosis

PAFR, Platelet activating factor receptor

greatly diminished in healthy semen

 
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